The aim of this study was to develop a computer simulation evaluating microvessel density
according to the Chalkley method on digital images taken from neovascular hot spots. An
image analysis algorithm has been developed using ImageJ, an extensible, open source
image processing and analysis software. The idea was to create a virtual Chalkley point
array graticule, and to calculate Chalkley counts automatically by stepwise angular rotation
of it on the superimposed images containing properly segmented microvessels. This …