[PDF][PDF] A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice

A Hayashi, T Sato, N Kamada, Y Mikami, K Matsuoka… - Cell host & …, 2013 - cell.com
A Hayashi, T Sato, N Kamada, Y Mikami, K Matsuoka, T Hisamatsu, T Hibi, A Roers…
Cell host & microbe, 2013cell.com
Imbalance in gut bacterial composition provokes host proinflammatory responses causing
diseases such as colitis. Colonization with a mixture of Clostridium species from clusters IV
and XIVa was shown to suppress colitis through the induction of IL-10-producing regulatory
T (Treg) cells. We demonstrate that a distinct Clostridium strain from cluster I, Clostridium
butyricum (CB), prevents acute experimental colitis in mice through induction of IL-10, an
anti-inflammatory cytokine. However, while CB treatment had no effect on IL-10 production …
Summary
Imbalance in gut bacterial composition provokes host proinflammatory responses causing diseases such as colitis. Colonization with a mixture of Clostridium species from clusters IV and XIVa was shown to suppress colitis through the induction of IL-10-producing regulatory T (Treg) cells. We demonstrate that a distinct Clostridium strain from cluster I, Clostridium butyricum (CB), prevents acute experimental colitis in mice through induction of IL-10, an anti-inflammatory cytokine. However, while CB treatment had no effect on IL-10 production by T cells, IL-10-producing F4/80+CD11b+CD11cint macrophages accumulated in the inflamed mucosa after CB treatment. CB directly triggered IL-10 production by intestinal macrophages in inflamed mucosa via the TLR2/MyD88 pathway. The colitis-preventing effect of CB was negated in macrophage-specific IL-10-deficient mice, suggesting that induction of IL-10 by intestinal macrophages is crucial for the probiotic action of CB. Collectively, CB promotes IL-10 production by intestinal macrophages in inflamed mucosa, thereby preventing experimental colitis in mice.
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