Transient suppression of MLH1 allows effective single-nucleotide substitution by single-stranded DNA oligonucleotides
M Dekker, S de Vries, M Aarts, R Dekker… - Mutation Research …, 2011 - Elsevier
Short synthetic single-stranded oligodeoxyribonucleotides (ssODNs) can be used to
introduce subtle modifications into the genome of mouse embryonic stem cells (ESCs). We
have previously shown that effective application of ssODN-mediated gene targeting in ESC
requires (transient) suppression of DNA mismatch repair (MMR). However, whereas
transient down-regulation of the mismatch recognition protein MSH2 allowed substitution of
3 or 4 nucleotides, 1 or 2 nucleotide substitutions were still suppressed. We now …
introduce subtle modifications into the genome of mouse embryonic stem cells (ESCs). We
have previously shown that effective application of ssODN-mediated gene targeting in ESC
requires (transient) suppression of DNA mismatch repair (MMR). However, whereas
transient down-regulation of the mismatch recognition protein MSH2 allowed substitution of
3 or 4 nucleotides, 1 or 2 nucleotide substitutions were still suppressed. We now …
