[HTML][HTML] CD74 is a survival receptor on colon epithelial cells
N Maharshak, S Cohen, F Lantner, G Hart… - World journal of …, 2010 - ncbi.nlm.nih.gov
World journal of gastroenterology: WJG, 2010•ncbi.nlm.nih.gov
AIM: To investigate the expression and function of CD74 in normal murine colon epithelial
cells (CEC) and colon carcinoma cells. METHODS: Expression of CD74 mRNA and protein
were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western
blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory
factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient
mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by …
cells (CEC) and colon carcinoma cells. METHODS: Expression of CD74 mRNA and protein
were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western
blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory
factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient
mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by …
Abstract
AIM: To investigate the expression and function of CD74 in normal murine colon epithelial cells (CEC) and colon carcinoma cells.
METHODS: Expression of CD74 mRNA and protein were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by (quantitative) qRT-PCR, RT-PCR, Western blotting and FACS.
RESULTS: CD74 was found to be expressed on normal CEC. Stimulation of CD74 by MIF induced a signaling cascade leading to up-regulation of Bcl-2 expression, resulting in a significant increased survival of CEC. CD74 was also expressed on the CT26 colon carcinoma cell line and its stimulation by MIF resulted in enhanced cell survival, up-regulation of Akt phosphorylation and Bcl-2 expression.
CONCLUSION: CD74 is expressed on CEC and colon carcinoma cells and serves as a survival receptor in these cells. These results may have implications on colorectal cancer research.
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